The PC-10 is compatible with various glass capillaries (e.g., borosilicate, aluminosilicate).
: Uses a combination of heater output and interchangeable weights (light and heavy). Operating Modes :
To fully leverage the capabilities of the Narishige PC-10, users should familiarize themselves with the manual's contents:
Keep the vertical slider tracks clean. Wipe them down with a lint-free cloth moistened with isopropyl alcohol. Do not use heavy oils, as they attract dust and cause friction. narishige pc10 manual new
The is a versatile, vertical-pull micropipette puller designed for the high-precision fabrication of glass microelectrodes and injection needles. This model replaces older semi-automatic units like the PP-830, offering a more automated and robust experience for researchers in biotechnology and electrophysiology. Core Operation Modes
Pulls the glass capillary in one continuous motion. This mode typically produces larger tip diameters or more "supple" pipettes.
: Heating and electromagnetic values are displayed as digital units (0–100), where a heater level of 100 corresponds to approximately 2.5V. This allows for reproducible mass production. The PC-10 is compatible with various glass capillaries (e
The heating coil activates based on the Heater 1 setting. The glass softens, and the slider drops slightly until it hits the intermediate micro-stop. This thins the glass middle section.
| Heat setting | Weight | Resulting pipette resistance (3 mm tip) | |--------------|--------|------------------------------------------| | 55 | 150g | No separation – too cold | | 62 | 150g | 2–3 MΩ (good for whole-cell patch) | | 68 | 150g | 5–7 MΩ (sharp intracellular) | | 75 | 200g | 10–12 MΩ (too long taper, fragile) | | 80+ | 100g | Blunt pipette – overheated glass |
So if you find one of those new community manuals? Treasure it. Print it. Laminate it. And maybe one day, pass it on to the next scientist who rescues a dusty PC-10 from the shelf. Wipe them down with a lint-free cloth moistened
Gradually increase the heater values by 2–3 units at a time. Capillary is off-center relative to the coil.
These settings allow the user to display and adjust the specific heating values for each pulling stage on the digital LED readout. Operational Parameters
The PC-10 allows for single-stage or two-stage pulling. The standard method for general-purpose intracellular electrodes is usually a single stage, while patch-clamp electrodes often benefit from a two-stage approach.
